Plant Cell & Tissue Culture In Vitro IV

(July 4-5, 2022)

Welcome to the 4th International Conference on “Plant Cell & Tissue Culture In Vitro”!

Plant cell, cultured in vitro, are able to regenerate into the complete fertile plants under the appropriate culture and environment conditions, e.g. plant cells are totipotent. Currently, the technology is not only the great tool in basic research, such as cell biology, genetics, biochemistry and biotechnology, but has also the direct commercial importance in the Mass Plant Micro-propagation, Genetic Transformation, Genome Editing, Speeding up Breeding Process via  production of Doubled Haploids, Production of Secondary Metabolites, etc.

The 4th International Conference on “Plant Cell & Tissue Culture In Vitroto be held in Vienna, Austria on July 4-5, 2022 will discuss wide range of modern in vitro plant cell and tissue culture technologies, fundamental aspects of plant cell totipotency, differentiation, regeneration, embryogenesis, and practical applications of plant cell in vitro technologies for crop improvement.

This two-day event will provide leading academy and industry scientists a platform to communicate recent advances in “Plant Cell & Tissue Culture In Vitro”, and an opportunity to establish multilateral collaboration.

The 4th International Conference on “Plant Cell & Tissue Culture  In Vitrowill cover the following research topics:

  • Plant Cell Totipotency & Differentiation
  • Plant Cell Organogenesis & Regeneration
  • Somatic Embryogenesis
  • Anther/Microspore Cultures (Androgenesis)
  • Ovary/Ovule Cultures (Gynogenesis)
  • Plant Cell Suspensions
  • Plant Cell Bioreactors
  • Protoplasts
  • Micropropagation
  • In Vitro Fertilization
  • Plant Transformation

Among the invited speakers are internationally known names such as:

Attila Fehér (Hungary), Christophe Gaillochet (Belgium), Pedro Pablo Gallego (Spain), Fredy Altpeter (USA), Pilar S Testillano (Spain), María Pilar Vallés (Spain), Ewa Grzebelus (Poland), Andreas Schiermeyer (Germany), Iwona Zur (Poland), Nieves Vidal (Spain), Klaus Palme (Germany), Agnieszka Szopa (Poland), Ralf Welsch (Germany), …

Approximately 250 participants are expected to attend this exciting scientific forum including almost 40 lectures delivered by worldwide known invited speakers and young, talented speakers selected from submitted abstracts. The program combines plenary lectures, poster sessions, exhibitions and interesting breakout sessions.

Deadline for submission of abstracts is June 10, 2022

Early bird registration: June 20, 2022

Please note: The next VISCEA Conference “Transgenic Plants & Transformation Technologies” will take place immediately after Plant Cell & Tissue Culture In vitro Conference on July 6-7, 2022 at the same location! We are pleased to offer you the chance to participate and register online for both at once for a very attractive registration fee!

We are looking forward to seeing you in Vienna on July 4-5, 2022!

Prof. Pedro Pablo Gallego (Spain, Conference Co-Organizer)
Prof. Alisher Touraev (VISCEA, Austria, Local Organizer)


Attila Fehér

Position Full professor; scientific advisor
Work Place Department of Plant Biology of the University of Szeged;
Biological Research Centre of the Eötvös Loránd Research Network
Research Interest plant morphogenesis and signal transduction



Pedro Pablo Gallego

Position Professor
Work Place Faculty of Biology | Vigo University | Campus Universitario | 36310 Vigo | Spain 
Research Interest



Nieves del Pilar Vidal González

Position Researcher
Work Place Department of Plant Production, Misión Biológica de Galicia, Sede de Santiago de Compostela (CSIC), Spain
Research Interest Biotechnology of woody plants



Pilar S. Testillano

Work Place Margarita Salas Center of Biological Research, CIB-CSIC
Research Interest Plant cell reprogramming, microspore embryogenesis



María-Pilar Vallés

Position Research scientist
Work Place Genetics and Plant Production Department. Aula Dei Experimental Station (EEAD-CSIC)
Research Interest Gametic embryogenesis
E-Mail vallé



Fredy Altpeter

Position Professor of Molecular Genetics and Biotechnology
Work Place University of Florida -IFAS
Research Interest Molecular Genetics and Biotechnology



Iwona Zur

Position Prof., the Head of Department of Cell Biology
Work Place Institute of Plant Physiology Polish Academy of Sciences
Research Interest doubled haploid technology; redox regulation; abiotic stress tolerance

(July 4-5, 2022)
July 4 (Monday)
08.00 – 17.00 Registration
09.00 – 09.20 Opening
Welcome address by Alisher Touraev (Local Chair, Austria)Welcome address by Pedro Pablo Gallego (Chair of the Conference, Spain)
09.10 – 09.45 (+5)

09.50 – 10.25 (+5)

Keynote Lecture 1: Attila Fehér (Hungary): Callus, Dedifferentiation, Totipotency, Somatic Embryogenesis: What These Terms Mean in the Era of Molecular Plant Biology?

Keynote Lecture 2: Klaus Palme (Germany): Innovative technology platform for in vitro cell studies: advantages in research and industrial applications

10.30 – 11.00 Coffee break
11.00 – 12.30 Session I: Plant Cell Totipotency & Differentiation
11.00 – 11.25 (+5) Christophe Gaillochet (Belgium): Control of plant cell fate transitions by transcriptional and hormonal signals
11.30 – 11.45 (+5) Lucie Fischerová (Czech Republic): Regeneration of Cannabis sativa (L.) in-vitro
11.50 – 12.05 (+5) Vera Martinez-Barradas (Chile): A Chilean common bean in vitro regeneration protocol to be implemented for CRISPR-Cas9 genetic edition
12.10 – 12.25 (+5) Maneea Moubarak (Italy): Extracellular vesicles from cell suspension culture of different explants of Solanum lycopersicum L.
12.30 – 14.00 Lunch + Poster Session (all numbers)
14.00 – 15.30 Session II: Plant Cell Organogenesis & Regeneration
14.00 – 14.20 (+5) Pedro Pablo Gallego (Spain): Machine Learning and Plant Tissue Culture Tools for Medicinal Plant Valorization
14.25 – 14.45 (+5) Nieves Vidal (Spain): Use of bioreactor systems in the propagation of forest trees
14.50 – 15.05 (+5) Elisabeth Kopper (Austria): Micropropagation and pathogen elimination i elderberry (Sambucus nigra L.)
15.10 – 15.30 (+5) Csaba Lantos (Hungary): Utilization and improvement of In Vitro Androgenesis in Cereal Breeding of CR LTD
15.35 – 16.00 Coffee break
16.00 – 17.30 Session III: In Vitro Propagation
16.00 – 16.25 (+5) Fredy Altpeter (USA): Overcoming Recalcitrance of Energycane to Tissue Culture and Genetic Transformation
16.30 – 16.50 (+5) Tobias Sieberer (Germany): Novel chemical strategies to boost shoot regeneration in recalcitrant crops
16.55 – 17.10 (+5) Marta Leonor Marulanda Angel (Colombia): In vitro propagation and somatic embryogenesis in Guadua angustifolia Kunth
17.15 – 17.35 (+5) Josef Baltazar ŠENKYŘÍK (Czech Republic): Astragalus membranaceus (Fisch.) – Artificial polyploidization: In vitro method for medical plants improvement.
17.35 – 19.00 Poster Session (all numbers) + Welcome Reception
19.00 – 22.00 Conference Dinner Party-Traditional Austrian food and wine, located in one of Vienna’s famous ‘Heurigen’
-Cost: 50,- EUR
July 5 (Tuesday)
08.00 – 17.00 Registration
09.00 – 10.30 Session IV: Haploid Technologies
09.00 – 09.20 (+5) Pilar S Testillano (Spain): Novel strategies with small molecules to improve microspore reprogramming to embryogenesis in crop and forest plants.
09.25 – 09.45 (+5) María Pilar Vallés (Spain): How Trichostatin A affects developmental reprogramming of bread wheat microspores
09.50 – 10.10 (+5) Iwona Zur (Poland): Reactive oxygen species and antioxidative defence as the determinants of microspore embryogenesis effectiveness
10.15 – 10.30 (+5) Yolanda Pérez Pérez (Spain): Endogenous auxin and cytokinin play key roles in stress-induced microspore embryogenesis of Brassica napus
10.35 – 11.00 Coffee break
11.00 – 12.30 Session V: Plant Protoplasts: Modern Applications
11.00 – 11.20 (+5) Ewa Grzebelus (Poland): Composition of the Reconstituted Cell Wall in Protoplast-Derived Cells of Daucus Is Affected by Phytosulfokine (PSK).
11.25 – 11.45 (+5) Ralf Welsch (Germany): Determination of Protoplast Growth Properties using Quantitative Single-Cell Tracking Analysis
11.50 – 12.10 (+5) Andreas Schiermeyer (Germany): Targeted insertion of large DNA sequences by homology-directed repair or non-homologous end joining in engineered tobacco BY-2 cells using designed zinc finger nucleases
12.15 – 12.35 (+5) Agnieszka Szopa (Poland): Production of Schisandra type lignans in in vitro cultures of S. chinensis, S. rubriflora and S. henryi
12.40 – 14.00 Lunch + Closing Ceremony